Cloned (Comment) | Organism |
---|---|
expression in HEK-293 cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K560E | mutation in the C-terminal basic motif. About 20% of wild-type activity | Homo sapiens |
additional information | deletion variant of the C-terminal part lacking amino acids 369-551. Whereas the C terminus binds to RNA, the deletion variant shows no shift of the RNA in EMSA experiments | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00092 | - |
(A)15 | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.00319 | - |
(A)15 | mutant K560E, pH 7.5, 37°C | Homo sapiens | |
0.00534 | - |
(A)15 | C-terminal deletion mutant, pH 7.5, 37°C | Homo sapiens | |
0.0469 | - |
ATP | mutant K560E, pH 7.5, 37°C | Homo sapiens | |
0.0636 | - |
ATP | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.0643 | - |
ATP | C-terminal deletion mutant, pH 7.5, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8NDF8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(A)15 + n ATP | in the presence of ATP, the incorporation of several nucleotides into the RNA substrate is observed | Homo sapiens | (A)15+n + n diphosphate | - |
? | |
ATP + 3' untranslated region of mRNA | - |
Homo sapiens | diphosphate + ? | - |
? | |
ATP + oligo(A)n | - |
Homo sapiens | diphosphate + oligo(A)n+1 | - |
? | |
ATP + oligo(U)n | - |
Homo sapiens | diphosphate + ? | - |
? | |
ATP + rRNA | - |
Homo sapiens | diphosphate + ? | - |
? | |
ATP + yeast tRNAiMet | in vitro-synthesized yeast tRNAiMet, but not the native tRNA is substrate | Homo sapiens | diphosphate + ? | - |
? | |
additional information | isoform PapD5 catalyzes the polyadenylation of different types of RNA substrates in vitro. PAPD5 is active without a protein cofactor. The C terminus of PpaD5 contains a stretch of basic amino acids that is involved in binding the RNA substrate. Incorporation of UTP, GTP, CTP is low and limited to single residues, showing a strong preference of isoform PAPD5 for ATP. No substrates: dNTPs | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PapD5 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
ATP | C-terminal deletion mutant, pH 7.5, 37°C | Homo sapiens | |
0.028 | - |
ATP | mutant K560E, pH 7.5, 37°C | Homo sapiens | |
0.097 | - |
ATP | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.142 | - |
(A)15 | mutant K560E, pH 7.5, 37°C | Homo sapiens | |
0.147 | - |
(A)15 | C-terminal deletion mutant, pH 7.5, 37°C | Homo sapiens | |
0.18 | - |
(A)15 | wild-type, pH 7.5, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.5 | - |
(A)15 | C-terminal deletion mutant, pH 7.5, 37°C | Homo sapiens | |
44.4 | - |
(A)15 | mutant K560E, pH 7.5, 37°C | Homo sapiens | |
195.7 | - |
(A)15 | wild-type, pH 7.5, 37°C | Homo sapiens |